供应GDNF重组人神经胶质源神经营养因子

【GDNF】重组人神经胶质源神经营养因子 
产品报价
 ￥800/2μg    ￥2080/10μg   ￥74880/1mg
 
背景资料
 GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
 
产品描述
 人神经胶质源神经营养因子重组蛋白是在大肠杆菌中重组表达的，是由两条非糖基化多肽链组成的同源二聚体。该重组蛋白包含有2 x 135个氨基酸，总分子量为30，360Da。该人神经胶质源神经营养因子重组蛋白较终由标准的色谱技术纯化出。
 
来源宿主
 大肠杆菌中重组表达
 
序列
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
 
溶解建议
 提供的人神经胶质源神经营养因子 (GDNF)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含人神经胶质源神经营养因子 (GDNF)的10mM柠檬酸钠和150mM NaCl溶液中经透析并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100μg/ml，后继实验过程中可根据实验要求进一步稀释。
 
保存建议
 人神经胶质源神经营养因子 (GDNF)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。